Protocol for positive control sample for 2d-dige

Box: Protocol for positive control sample for 2d-dige

Taken from: Application of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics

Published in: Nature Protocols

DIGE stands for difference gel electrophoresis.




Typical 2D images of the proteins extracted from a 1 mm2 area of (a) liver cancer tissue and (b) lung adenocarcinona tissue.

Freezing and shipping skin-derived precursors (SKPs)

Box: Freezing and shipping skin-derived precursors (SKPs)

Taken from: Isolation of skin-derived precursors (SKPs) and differentiation and enrichment of their Schwann cell progeny

Published in: Nature Protocols

Regeneration test of three rice varieties

Image Title: Regeneration test of three rice varieties. (a) A japonica variety, "Nipponbare," that has a high-regeneration frequency. (b) A japonica variety, "Koshihikari," that has a low-regeneration frequency. (c) An indica variety, "Kasalath," that exhibits a high-regeneration frequency.

Taken from: A protocol for Agrobacterium-mediated transformation in rice

Transformation of Agrobacterium

Box: Transformation of Agrobacterium

From: Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants

Published in: Nature Protocols

YEB medium Yeast extract (1.0 g l^-1), beef extract (5.0 g l^-1), peptone (5.0 g l^-1), sucrose (5.0 g l^-1), MgSO₄7H₂O (0.5 g l^-1), pH 7.0. Also include the proper antibiotic resistance selection drug for the FP fusion construct carried by these bacteria.

The Boxed protocol is for a reagent used in the Nature Protocol. It is based on the method described in Storage of competent cells for Agrobacterium transformation.

Quantifying pollen viability in Arabidopsis

Box: Quantifying pollen viability

From: Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets

Published in: Nature Protocols

The PGM solution used in the Boxed protocol is prepared as follows:
To 950 ml H₂O, add 170 g sucrose (17% wt/vol), 2 ml 1 M CaCl₂ (2 mM), 3.25 ml 0.5 M boric acid (1.625 mM) and 100 mul Triton-X (0.1% vol/vol).

Deriving Linkage Distance and Gene Order From Three-Point Crosses contains some background information about the ABC genotype reference...

BIO-RAD protein assay

Box: BIO-RAD protein assay

Taken From: Southwestern blotting in investigating transcriptional regulation

Published in: Nature Protocols

This is a short protocol for determining protein concentrations.
The main protocol is open access during 2008 being part of the sample issue.

Preparation of the per-O-trimethylsilylated glucoside 1a and 1b

Box: Preparation of the per-O-trimethylsilylated glucoside 1a and 1b

Taken from: Regioselective one-pot protection of glucose

Published in: Nature Protocols

Glucosides 1a and 1b, shown in Figure 1 of the protocol, are the starting materials for a series of reactions to protect different combinations of hydroxyl groups in the glucose molecule.