Box: Preparation of bovine thymocytes
Taken from: Flow cytometry and FISH to measure the average length of telomeres (flow FISH)
Published in: Nature Protocols
I really like the first step in the boxed method: "Arrange for fresh bovine thymus through a local meat packer. Request the collection of 2–3 small (e.g., cubic inch) pieces in a 1,000 ml container filled with digest solution. Arrange for pickup and process immediately."
FISH is another one of these very clever acronyms that make it so that if you search for them in PubMed or Scopus (or anywhere else for that matter) you are initially puzzled by the presence of large numbers of articles that are completely uninteresting to you. In this case FISH stands for fluorescent in situ hybridization.
[moderately amusing aside: the first search that I EVER did on Medline was for PET (as in positron emission tomography), but of course I got quite a lot more than that...]
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