Quantifying pollen viability in Arabidopsis

Box: Quantifying pollen viability

From: Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets

Published in: Nature Protocols

The PGM solution used in the Boxed protocol is prepared as follows:
To 950 ml H₂O, add 170 g sucrose (17% wt/vol), 2 ml 1 M CaCl₂ (2 mM), 3.25 ml 0.5 M boric acid (1.625 mM) and 100 mul Triton-X (0.1% vol/vol).

Deriving Linkage Distance and Gene Order From Three-Point Crosses contains some background information about the ABC genotype reference...