Preparation of celery juice extract

Box: Preparation of celery juice extract

Taken from: A protocol for TILLING and Ecotilling in plants and animals

Published in: Nature Protocols

TILLING and Ecotilling sound very agricultural, but (possibly disappointingly) TILLING actually stands for "Targeting-Induced Local Lesions IN Genomes".
So why would you (ever) want to make a celery juice extract? It turns out that celery juice contains an enzyme, a single-strand-specific nuclease, and the extract is used as a reagent in the TILLING protocol.
Here is a picture showing its activity:


Gel image showing the effect of the amount of celery juice extract used to cleave mismatches




This figure was originally published in Nucleic Acids Research.