Box: Transformation of Agrobacterium
From: Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
Published in: Nature Protocols
YEB medium Yeast extract (1.0 g l-1), beef extract (5.0 g l-1), peptone (5.0 g l-1), sucrose (5.0 g l-1), MgSO47H2O (0.5 g l-1), pH 7.0. Also include the proper antibiotic resistance selection drug for the FP fusion construct carried by these bacteria.
The Boxed protocol is for a reagent used in the Nature Protocol. It is based on the method described in Storage of competent cells for Agrobacterium transformation.
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